Our Services
Divergene works closely with CDMOs and gene therapy companies to support their next-generation sequencing capability from upstream vector engineering to downstream vector QC
End-to-end Sequencing
Whether you are looking for AAV capsid library enrichment or interested in post-infection solutions, Divergene utilizes the latest technologies on the market, including PacBio® long-read (>10kb) and Illumina® short-read sequencing, to provide custom, in-depth solutions for complex AAV gene therapy applications.
- AAV Sequencing
- Vector Discovery
- Confirm Plasmid Sequences
- Quantify AAV Expression
- Validate Packaged Material
- Monitor Host Response
- Single Cell Sequencing
- Transcriptome Analysis
If any of the applications interest you, get in touch with us or
Order on Science Exchange.
AAV GENOME SEQUENCING WORKFLOW
Divergene solutions streamline AAV cell and gene therapy research by providing a comprehensive approach. These solutions are optimized for adeno-associated virus (AAV) applications and include a proprietary QC process. With Divergene, researchers can easily verify the integrity of packaged AAV vectors, measure AAV expression post-infection, monitor host response, and more. Divergene’s technologies, such as PacBio® long-read (>10kb) and Illumina® short-read sequencing, are used to provide customized and in-depth solutions for complex AAV gene therapy applications.
QC & Library Preparation
Sequencing
Bioinformatics Analysis
Custom NGS Analysis Pipeline Development
We’re experts on bioinformatics software; if you want something built, you probably want us. And we will deliver.
If you answered yes to one or more of these questions, get in touch with us or
Order on Science Exchange.
Why Choose Us
Superior Speed & Data Quality
Robust framework, stringent quality control producing high-quality data
Ph.D.-Level Support
From start to finish, our experienced team provides insight and responses within hours
Customized solutions
From wet lab to dry lab, we provide support at every level and actionable dashboards/reports
cell & gene therapy focus
Trusted partner for top cell and gene therapy biotechs and biopharma
NGS PLATFORMS
Divergene utilizes Illumina and other cutting-edge NGS platforms. For information on our NGS platforms as well as recommended configurations of your projects, please contact us.
TECHNICAL RESOURCES
PUBLICATION
Adeno-associated Virus Genome Population Sequencing Achieves Full Vector Genome Resolution and Reveals Human-Vector Chimeras
Discover how researchers utilized single molecule, real-time (SMRT®) sequencing to profile rAAV-packaged genomes and assess full-length integrity for rAAV quality control.
Publication
Direct ITR-to-ITR Nanopore Sequencing of AAV Vector Genomes
Nanopore sequencing is a quick and low-cost alternative that can obtain full-length resolution of encapsidated genomes, although with low accuracy and high miscalling rates.
PUBLICATION
Advanced Characterization of DNA Molecules in rAAV Vector Preparations by Single-stranded Virus Next-generation Sequencing
In this study, learn how short-read sequencing technology can extensively characterize the rAAV genome while simultaneously detecting the presence of DNA contaminants.
Publication
Human and Insect Cell-Produced Recombinant Adeno-Associated Viruses Show Differences in Genome Heterogeneity
The exact genome compositions of full, intermediate, and empty capsids remain unknown, but recent AAV-genome sequencing reveals surprising findings on vector efficacy and safety.