Leverage sequencing and deep learning algorithms to accurately characterize your AAV product
Sequencing plays a crucial role in characterizing adeno-associated virus (AAV) vectors, providing essential insights into their genetic makeup, quality, and functionality. AAV sequencing encompasses various applications.
AAV Genome Sequencing Workflow
Divergene solutions offer a streamlined approach to AAV cell and gene therapy (CGT) research, designed to optimize adeno-associated virus (AAV) applications. Our proprietary QC process ensures the integrity of packaged AAV vectors. Researchers can rely on Divergene to measure AAV expression post-infection, monitor host responses, and more. We employ cutting-edge technologies, including PacBio® long-read (>10kb) and Illumina® short-read sequencing, to provide customized and comprehensive solutions tailored to complex AAV gene therapy applications. Many CGT companies choose Divergene for efficient and effective AAV research solutions.
QC & Library Preparation
Sequencing
Bioinformatics Analysis
Flyer
AAV genome sequencing with SMRT sequencing
Sample Type, Sample Quantity, Platform, Limit of Detection and Turn-around Time
Technical resources
PUBLICATION
Adeno-associated Virus Genome Population Sequencing Achieves Full Vector Genome Resolution and Reveals Human-Vector Chimeras
Discover how researchers utilized single molecule, real-time (SMRT®) sequencing to profile rAAV-packaged genomes and assess full-length integrity for rAAV quality control.
Publication
Direct ITR-to-ITR Nanopore Sequencing of AAV Vector Genomes
Nanopore sequencing is a quick and low-cost alternative that can obtain full-length resolution of encapsidated genomes, although with low accuracy and high miscalling rates.
PUBLICATION
Advanced Characterization of DNA Molecules in rAAV Vector Preparations by Single-stranded Virus Next-generation Sequencing
In this study, learn how short-read sequencing technology can extensively characterize the rAAV genome while simultaneously detecting the presence of DNA contaminants.
Publication
Human and Insect Cell-Produced Recombinant Adeno-Associated Viruses Show Differences in Genome Heterogeneity
The exact genome compositions of full, intermediate, and empty capsids remain unknown, but recent AAV-genome sequencing reveals surprising findings on vector efficacy and safety.